mouse sam library Search Results


mouse sam library  (Addgene inc)
92
Addgene inc mouse sam library
Mouse Sam Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse sam library/product/Addgene inc
Average 92 stars, based on 1 article reviews
mouse sam library - by Bioz Stars, 2026-03
92/100 stars
  Buy from Supplier
crispr activation system  (Addgene inc)
93
Addgene inc crispr activation system
a . A375 shFluc or shLIMIT cells were treated with IFNγ for 24 hours. RNA levels of LIMIT were determined by qRT-PCR. P value by 2-sided t-test. b . A375 shFluc or shLIMIT cells were treated with IFNγ for the indicated time. Protein levels of phospho-STAT1 (p-Y701), STAT1, and GAPDH were determined by Western blotting. 1 of 2 experiments is shown. c . A375 shFluc or shLIMIT cells were treated with IFNγ for 48 hours. Surface expression of HLA-ABC were determined by flow cytometry (FACS). P value by 2-sided t-test. d-g . YUMM1.7 ( d, e ) or CT26 ( f, g ) cells carrying shFluc or shLimit were treated with IFNγ. RNA levels of Limit were determined 24 hours after treatment ( d, f ). Surface staining of MHC-I (H2-D b ) was detected 48 hours after treatment ( e, g ). P value by 2-sided t-test. h-i . A375 WT or LIMIT promoter deletion cells were treated with IFNγ. RNA levels of LIMIT were determined 24 hours after treatment ( h ). Surface expression of HLA-ABC were determined 48 hours after treatment ( i ). P value by 2-sided t-test. j-k . B16 cells <t>were</t> <t>transfected</t> with dCas9-VPR, together with non-targeting sgRNA (sgNT) or sgRNA targeting the promoter of Limit (sgLimit). RNA levels of Limit were determined 24 hours after treatment ( j ). Surface expression of MHC-I (H2-D b ) or PD-L1 were determined 48 hours after treatment ( k ). P value by 2-sided t-test. l . B16-OVA cells carrying shFluc or shB2m were manipulated with Limit <t>CRISPRa</t> (sgLimit) for 48 hours. Surface expression of OVA-H2K b were determined by FACS. P value by 2-sided t-test. m-n . B16-OVA cells were manipulated with B2m knocking down (shB2m) or Limit CRISPRa (sgLimit), and co-cultured with OT-I cell at the ratio of 1:4. Cell death was measured by PI staining in CD45 − tumor cells. Dot plots ( m ) and statistical results ( n ) are shown. P value by 2-sided t-test. All data are mean ± SD. n = 3 biological independent samples in ( a, c, d, e, f, g, h, i, j, k, l, n ). Source data are provided in Soure_data_Fig2.xlsx and Unmodified_blots_Fig2.pdf.
Crispr Activation System, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr activation system/product/Addgene inc
Average 93 stars, based on 1 article reviews
crispr activation system - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier

Image Search Results


a . A375 shFluc or shLIMIT cells were treated with IFNγ for 24 hours. RNA levels of LIMIT were determined by qRT-PCR. P value by 2-sided t-test. b . A375 shFluc or shLIMIT cells were treated with IFNγ for the indicated time. Protein levels of phospho-STAT1 (p-Y701), STAT1, and GAPDH were determined by Western blotting. 1 of 2 experiments is shown. c . A375 shFluc or shLIMIT cells were treated with IFNγ for 48 hours. Surface expression of HLA-ABC were determined by flow cytometry (FACS). P value by 2-sided t-test. d-g . YUMM1.7 ( d, e ) or CT26 ( f, g ) cells carrying shFluc or shLimit were treated with IFNγ. RNA levels of Limit were determined 24 hours after treatment ( d, f ). Surface staining of MHC-I (H2-D b ) was detected 48 hours after treatment ( e, g ). P value by 2-sided t-test. h-i . A375 WT or LIMIT promoter deletion cells were treated with IFNγ. RNA levels of LIMIT were determined 24 hours after treatment ( h ). Surface expression of HLA-ABC were determined 48 hours after treatment ( i ). P value by 2-sided t-test. j-k . B16 cells were transfected with dCas9-VPR, together with non-targeting sgRNA (sgNT) or sgRNA targeting the promoter of Limit (sgLimit). RNA levels of Limit were determined 24 hours after treatment ( j ). Surface expression of MHC-I (H2-D b ) or PD-L1 were determined 48 hours after treatment ( k ). P value by 2-sided t-test. l . B16-OVA cells carrying shFluc or shB2m were manipulated with Limit CRISPRa (sgLimit) for 48 hours. Surface expression of OVA-H2K b were determined by FACS. P value by 2-sided t-test. m-n . B16-OVA cells were manipulated with B2m knocking down (shB2m) or Limit CRISPRa (sgLimit), and co-cultured with OT-I cell at the ratio of 1:4. Cell death was measured by PI staining in CD45 − tumor cells. Dot plots ( m ) and statistical results ( n ) are shown. P value by 2-sided t-test. All data are mean ± SD. n = 3 biological independent samples in ( a, c, d, e, f, g, h, i, j, k, l, n ). Source data are provided in Soure_data_Fig2.xlsx and Unmodified_blots_Fig2.pdf.

Journal: Nature cell biology

Article Title: LIMIT is an immunogenic lncRNA in cancer immunity and immunotherapy

doi: 10.1038/s41556-021-00672-3

Figure Lengend Snippet: a . A375 shFluc or shLIMIT cells were treated with IFNγ for 24 hours. RNA levels of LIMIT were determined by qRT-PCR. P value by 2-sided t-test. b . A375 shFluc or shLIMIT cells were treated with IFNγ for the indicated time. Protein levels of phospho-STAT1 (p-Y701), STAT1, and GAPDH were determined by Western blotting. 1 of 2 experiments is shown. c . A375 shFluc or shLIMIT cells were treated with IFNγ for 48 hours. Surface expression of HLA-ABC were determined by flow cytometry (FACS). P value by 2-sided t-test. d-g . YUMM1.7 ( d, e ) or CT26 ( f, g ) cells carrying shFluc or shLimit were treated with IFNγ. RNA levels of Limit were determined 24 hours after treatment ( d, f ). Surface staining of MHC-I (H2-D b ) was detected 48 hours after treatment ( e, g ). P value by 2-sided t-test. h-i . A375 WT or LIMIT promoter deletion cells were treated with IFNγ. RNA levels of LIMIT were determined 24 hours after treatment ( h ). Surface expression of HLA-ABC were determined 48 hours after treatment ( i ). P value by 2-sided t-test. j-k . B16 cells were transfected with dCas9-VPR, together with non-targeting sgRNA (sgNT) or sgRNA targeting the promoter of Limit (sgLimit). RNA levels of Limit were determined 24 hours after treatment ( j ). Surface expression of MHC-I (H2-D b ) or PD-L1 were determined 48 hours after treatment ( k ). P value by 2-sided t-test. l . B16-OVA cells carrying shFluc or shB2m were manipulated with Limit CRISPRa (sgLimit) for 48 hours. Surface expression of OVA-H2K b were determined by FACS. P value by 2-sided t-test. m-n . B16-OVA cells were manipulated with B2m knocking down (shB2m) or Limit CRISPRa (sgLimit), and co-cultured with OT-I cell at the ratio of 1:4. Cell death was measured by PI staining in CD45 − tumor cells. Dot plots ( m ) and statistical results ( n ) are shown. P value by 2-sided t-test. All data are mean ± SD. n = 3 biological independent samples in ( a, c, d, e, f, g, h, i, j, k, l, n ). Source data are provided in Soure_data_Fig2.xlsx and Unmodified_blots_Fig2.pdf.

Article Snippet: To apply CRISPR activation system to activate mouse Limit, phU6-sgRNAs were transfected together with SP-dCas9-VPR (Addgene #63798) into B16 cells.

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Flow Cytometry, Staining, Transfection, Cell Culture

a . Co-IP of GBP1-5 with HSP90 antibody in IFNγ-pretreated A375 cells. 1 of 3 experiments is shown. b . Co-IP of HSP90 with Flag antibody in Flag-GBP1-overexpressed A375 cells. * indicated the band shift of HSP90 upon GBP1 overexpression. 1 of 2 experiments is shown. c . Immunofluorescence staining of GBP1 and HSP90 in IFNγ-pretreated A375 cells. 1 of 4 images is shown. d . 293T cells were forced expression of Flag-HSF1 and increased doses of Flag-GBP1. Co-IP of HSF1 or GBP1 with HSP90 antibody were performed 24 hours afterwards. 1 of 2 experiments is shown. e . A375 cells were treated with HSP90 inhibitor, or forced expression of GBP1. Indicated proteins were detected 48 hours afterwards. 1 of 2 experiments is shown. f-g . A375 cells were treated with IFNγ and KRIBB11. RNA ( f ) or surface staining ( g ) levels of indicated genes were determined 48 hours afterwards. P value by 2-sided t-test. h . YUMM1.7 shFluc or shHsf1 cells were treated with IFNγ. Total protein ( h ) or surface expression ( i ) levels of indicated genes were determined 48 hours afterwards. 1 of 2 experiments is shown. P value by 2-sided t-test. j-k . Tumor growth curves of YUMM1.7 shFluc or shHsf1 cells in NSG mice ( j ) or wild type C57BL/6 mice ( k ). n = 5 ( j ) or 6 ( k ) animals, P value by 2-sided t-test for end point tumor volume. l . Percentages of CD3 + , Ki67 + , IFNγ + , and TNFα + T cells in YUMM1.7 shFluc or shHsf1 tumors. n = 5 biological independent samples, P value by 2-sided t-test. m . A375 shFluc or shLIMIT cells were transfected with HSE-luc and PRL-SV40 overnight, and then treated with IFNγ for additional 48 hours. HSF1 transcriptional activity is depicted as the relative luciferase activity. P value by 2-sided t-test. n . B16 cells were manipulated with Limit CRISPRa and treated with KRIBB11. Surface expression of MHC-I (H2-D b ) was determined 48 hours afterwards. P value by 2-sided t-test. All data are mean ± SD. n = 3 biological independent samples in ( f, g, i, m, n ). Source data are provided in Soure_data_Fig6.xlsx and Unmodified_blots_Fig6.pdf.

Journal: Nature cell biology

Article Title: LIMIT is an immunogenic lncRNA in cancer immunity and immunotherapy

doi: 10.1038/s41556-021-00672-3

Figure Lengend Snippet: a . Co-IP of GBP1-5 with HSP90 antibody in IFNγ-pretreated A375 cells. 1 of 3 experiments is shown. b . Co-IP of HSP90 with Flag antibody in Flag-GBP1-overexpressed A375 cells. * indicated the band shift of HSP90 upon GBP1 overexpression. 1 of 2 experiments is shown. c . Immunofluorescence staining of GBP1 and HSP90 in IFNγ-pretreated A375 cells. 1 of 4 images is shown. d . 293T cells were forced expression of Flag-HSF1 and increased doses of Flag-GBP1. Co-IP of HSF1 or GBP1 with HSP90 antibody were performed 24 hours afterwards. 1 of 2 experiments is shown. e . A375 cells were treated with HSP90 inhibitor, or forced expression of GBP1. Indicated proteins were detected 48 hours afterwards. 1 of 2 experiments is shown. f-g . A375 cells were treated with IFNγ and KRIBB11. RNA ( f ) or surface staining ( g ) levels of indicated genes were determined 48 hours afterwards. P value by 2-sided t-test. h . YUMM1.7 shFluc or shHsf1 cells were treated with IFNγ. Total protein ( h ) or surface expression ( i ) levels of indicated genes were determined 48 hours afterwards. 1 of 2 experiments is shown. P value by 2-sided t-test. j-k . Tumor growth curves of YUMM1.7 shFluc or shHsf1 cells in NSG mice ( j ) or wild type C57BL/6 mice ( k ). n = 5 ( j ) or 6 ( k ) animals, P value by 2-sided t-test for end point tumor volume. l . Percentages of CD3 + , Ki67 + , IFNγ + , and TNFα + T cells in YUMM1.7 shFluc or shHsf1 tumors. n = 5 biological independent samples, P value by 2-sided t-test. m . A375 shFluc or shLIMIT cells were transfected with HSE-luc and PRL-SV40 overnight, and then treated with IFNγ for additional 48 hours. HSF1 transcriptional activity is depicted as the relative luciferase activity. P value by 2-sided t-test. n . B16 cells were manipulated with Limit CRISPRa and treated with KRIBB11. Surface expression of MHC-I (H2-D b ) was determined 48 hours afterwards. P value by 2-sided t-test. All data are mean ± SD. n = 3 biological independent samples in ( f, g, i, m, n ). Source data are provided in Soure_data_Fig6.xlsx and Unmodified_blots_Fig6.pdf.

Article Snippet: To apply CRISPR activation system to activate mouse Limit, phU6-sgRNAs were transfected together with SP-dCas9-VPR (Addgene #63798) into B16 cells.

Techniques: Co-Immunoprecipitation Assay, Electrophoretic Mobility Shift Assay, Over Expression, Immunofluorescence, Staining, Expressing, Transfection, Activity Assay, Luciferase